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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab <t>2020a</t> and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Tissue perfusion during hemodialysis. SAD images from an IVVM observation of the EDL microvasculature at different timepoints during HD (tissue area of 233 µm × 373 µm). ( A ): Baseline SAD image. ( B ): SAD image at 1 h sham procedure. ( C ): SAD image at 1 h hemodialysis procedure. ( D ): SAD image at 2 h hemodialysis (see the associated inverse videos in supplemental data: -Fig. 3A, -Fig. 3B, -Fig. 3C and -Fig. 3D) (image produced by B(GH) Janssen using Matlab <t>2020a,</t> the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).
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Tissue perfusion during hemodialysis. SAD images from an IVVM observation of the EDL microvasculature at different timepoints during HD (tissue area of 233 µm × 373 µm). ( A ): Baseline SAD image. ( B ): SAD image at 1 h sham procedure. ( C ): SAD image at 1 h hemodialysis procedure. ( D ): SAD image at 2 h hemodialysis (see the associated inverse videos in supplemental data: -Fig. 3A, -Fig. 3B, -Fig. 3C and -Fig. 3D) (image produced by B(GH) Janssen using Matlab <t>2020a,</t> the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).
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Tissue perfusion during hemodialysis. SAD images from an IVVM observation of the EDL microvasculature at different timepoints during HD (tissue area of 233 µm × 373 µm). ( A ): Baseline SAD image. ( B ): SAD image at 1 h sham procedure. ( C ): SAD image at 1 h hemodialysis procedure. ( D ): SAD image at 2 h hemodialysis (see the associated inverse videos in supplemental data: -Fig. 3A, -Fig. 3B, -Fig. 3C and -Fig. 3D) (image produced by B(GH) Janssen using Matlab <t>2020a,</t> the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).
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2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Life Sciences

Article Title: Glycolytic inhibitor 2-deoxy- d -glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions

doi: 10.1016/j.lfs.2022.120411

Figure Lengend Snippet: 2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For this purpose, the study employed algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a (Mathworks).

Techniques: Virus, Infection, Fluorescence, Suspension, Derivative Assay, Two Tailed Test

Tissue perfusion during hemodialysis. SAD images from an IVVM observation of the EDL microvasculature at different timepoints during HD (tissue area of 233 µm × 373 µm). ( A ): Baseline SAD image. ( B ): SAD image at 1 h sham procedure. ( C ): SAD image at 1 h hemodialysis procedure. ( D ): SAD image at 2 h hemodialysis (see the associated inverse videos in supplemental data: -Fig. 3A, -Fig. 3B, -Fig. 3C and -Fig. 3D) (image produced by B(GH) Janssen using Matlab 2020a, the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).

Journal: Scientific Reports

Article Title: Intravital microscopic observation of the microvasculature during hemodialysis in healthy rats

doi: 10.1038/s41598-021-03681-2

Figure Lengend Snippet: Tissue perfusion during hemodialysis. SAD images from an IVVM observation of the EDL microvasculature at different timepoints during HD (tissue area of 233 µm × 373 µm). ( A ): Baseline SAD image. ( B ): SAD image at 1 h sham procedure. ( C ): SAD image at 1 h hemodialysis procedure. ( D ): SAD image at 2 h hemodialysis (see the associated inverse videos in supplemental data: -Fig. 3A, -Fig. 3B, -Fig. 3C and -Fig. 3D) (image produced by B(GH) Janssen using Matlab 2020a, the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).

Article Snippet: All images were processed using the in-house written Matlab based software, (Matlab 2020a, the Matworks Inc, Natrick, MA USA; https://www.mathworks.com ).

Techniques: Produced